The present work investigates the efficiency of Paecilomyces variotii upon degrading Reactive Black dye which has been termed a recalcitrant variety of synthetic dye. In this research, initially a predominant fungal species, Paecilomyces variotii was isolated from the tannery effluent sample. The study was carried out by assessing the ability of fungi to decolorize the dye under various parameters like pH (5,7 and 9), Temperature (7°C, 30°C, and 45°C), Dye concentration (200, 300, and 400 mg/L) for different incubation or exposure time interval (3, 5 and 7 days). From the experimental study, it was found that Paecilomyces variotii showed a maximum percentage of dye decolorization at 7°C at pH 9 with 75%, at 30°C at pH 7 with 85%, at 45°C at pH 5 with 82% and a maximum period of incubation with 7 days in 200 mg.L-1 concentration. This result conveys that the strength of Paecilomyces variotii in decolorizing the synthetic dye is effective at a moderate temperature with neutral pH for maximum exposure time. So Paecilomyces variotii could be a good candidate of choice for the biodegradation of various synthetic dyes when manipulated wisely. Also, the result sparks a positive attribute toward decreasing industrial wastewater pollution.

Introduction and Aim: Presently, research and development in nanomaterials is gaining hypersonic reach in various areas of applications.  Biological way of preparing such nanomaterials is acquiring noteworthiness in the view of affordability and environment-friendly approach. In this current work, gold nanoparticles(AuNPs) were prepared using leaves extract of Chloroxylon swietenia having anticancer behaviour with the focus to blend the therapeutic activity among the nanoparticles was studied by conjugating with 5 Fluorouracil (5-FU) to target breast cancer cells.   Materials and Methods: Chloroxylon swietenia leaf extract was prepared and chloroauric acid (HAuCl4) was added to synthesis AuNPs. As prepared AuNPs were characterized using UV-visible spectrophotometry, TEM and EDAX. The antioxidant tests were done by DPPH assay. Further, the AuNPs were conjugated with5-fluorouracil. The in vitro cytotoxicity of the synthesized gold nanoparticles and conjugated AuNPs were assessed by MTT assay against normal and A549 breast cancer cell lines and the efficacy was noted.   Results: Preparation of AuNPsthrough the leaf extract of C. swietenia was carried out in an effective way.The preliminary test for the confirmation of as synthesized NPs was done through spectroscopic analysis, resulting in a characteristic peak at 539nm. The TEM examination provided the information about size and the pattern of NPs. Cytotoxicity investigation of NPs with VERO cell lines confirmed its non-toxic nature towards the cells. Anticancer study with A549 breast cancer cell lines also proved the NPs with IC50 value at 25µl/ml.    Conclusion: The conjugated AuNPs with 5-FU revealed anticancer activity in A549 breast cancer cell line but correspondingly it is proved to be safe with standard VERO cell line. The findings stand with a strong point to expand the studies through the evolution of potential drug molecules to deal with the disease of interest.

Background: From time immemorial herbal preparations are been employed for the treatment of several ailments. In recent years due to poor bioavailability the conventional herbal preparations are replaced by phytoniosomes, an advanced novel drug delivery system in which the herbal extracts are incorporated into a non-ionic surfactant to yield higher absorption and remarkable desired pharmacological activity. The present study is aimed to prepare and characterize the ethanolic leaf extract of Tinospora cordifolia (nELETC) loaded phytoniosome and to compare its antioxidant properties with ethanolic leaf extract of Tinospora cordifolia (ELETC). Methods: The ethanolic leaf extract and ethanolic leaf extract of Tinospora cordifolia loaded phytoniosome (ELETC and nELETC) were prepared. The characterization of the prepared phytoniosomes were performed by UV-Visible spectroscopy, FTIR, XRD, SEM, TEM, DLS and zeta potential. The nontoxic nature of the prepared phytoniosomes was analyzed using MTT assay in vero cell line. The antioxidant potential of ELETC and nELETC were compared by the scavenging activity of DPPH, Hydrogen peroxide and Superoxide radicals. Results: The formation of ethanolic leaf extract of Tinospora cordifolia loaded phytoniosome (nELETC) was confirmed with UV-Vis spectroscopy. The SEM and TEM images confirmed the spherical shape of the nELETC with average size ranging from 600 to 1800 nm. The zeta potential showed magnitude of -65.55 to -77.83 mV and its crystalline structure was confirmed by XRD analysis. Through the FTIR spectrum presence of alcohols, alkanes, phenols, esters, aliphatic and aromatic compounds as well as alkenes and carbolic acids were identified. MTT assay establishes the non-toxic nature of the synthesized nELETC and excellent antioxidant potential was observed for nELETC than ELETC. Conclusion: In conclusion, the ethanolic leaf extract of Tinospora cordifolia loaded phytoniosome (nELETC) will serve as a promising drug carrier in scavenging the free radicals and can be used in various biological applications.

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