Although the concept of Reverse Vaccinology was first pioneered for sepsis and meningococcal meningitidis causing bacterium, Neisseria meningitides, no broadly effective vaccine against serogroup B meningococcal disease is yet available. In the present investigation, HLA distribution analysis was undertaken to select three most promiscuous T-cell epitopes out of ten computationally validated epitopes of Iron acquisition proteins from Neisseria MC58 by using the population coverage tool of Immune Epitope Database (IEDB). These epitopes have been determined on the basis of their binding ability with maximum number of HLA alleles along with highest population coverage rate values for all the geographical areas studied. The comparative population coverage analysis of moderately immunogenic and high immunogenic peptides suggests that the former may activate T-cell response in a fairly large proportion of people in most geographical areas, thus indicating their potential for development of epitope-based vaccine.

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Aims and objective:To assess the feasibility of singleincision laparoscopic cholecystectomy (SILC) with conventional laparoscopic instruments and to compare it with four-port conventional laparoscopic cholecystectomy (LC) regarding various intraoperative and postoperative factors. Materials and methods:This is a prospective randomized controlled study carried out at Santosh Medical College and Hospitals, Ghaziabad from March 2014 to September 2015. This study included 60 patients with cholelithiasis who were divided into two groups of 30 patients each. Group I was offered four-port conventional LC and group II underwent SILC. Results and observations:Cholelithiasis was commonly seen in young females. Single-incision laparoscopic cholecystectomy took more operating time than conventional LC due to more operative difficulty. Outcome of SILC was 79.6% (23 of 30). However, postoperative complications and pain (measured by visual analog scale scoring system) were almost the same in both groups. Cosmetic outcome was better in SILC group. Conclusion:Single-incision laparoscopic cholecystectomy has no added advantage over conventional LC, but it can be performed in selected patients for better cosmetic results.

Introduction: Kocuria kristinae is a commensal organism, sometimes considered as a lab contaminant, but its repeated isolation from clinical samples in immunocompromised patients should raise red flags. Materials and Methods: We confirmed the infection with re-isolation of the organism from the same site before starting treatment. For the identification of Kocuria kristinae we used IDGP cards on VITEK 2 compact system. Antibiotic susceptibility test was done manually following CLSI guidelines 2018 for Coagulase-negative staphylococci. Results: A total of 510 major head neck oncosurgeries were performed during the period of two years. Out of which 120 patients had skin and soft tissue infections. Out of these infected patients, 90 were culture positive and of these Kocuria kristinae were isolated in 12 patients. Resistance to penicillin and oxacillin is seen in all isolates. Conclusion: Kocuria kristinae should not be ignored as a commensal flora or lab contaminant in immunocompromised hosts. Its Increase in resistance pattern is a matter of concern. It is an ignored opportunistic pathogen whose detailed sensitivity test should be developed to treat patients timely and effectively.