Background Recent advances in the diversified anti-diabetic drugs have appeared in the startling increase in the count of poisoning cases. The epidemics of diabetes mellitus are increasing; hence, the no. of anti-diabetic drug users raised by 42.9%. The use of glimepiride raised to 24%. As the toxicity and drug cases are also escalating with increasing epidemics of diabetes mellitus, a novel gas chromatography-mass spectrometry (GC-MS) method for detecting glimepiride in biological matrices is developed. Results Liquid-liquid extraction method was employed by using 1-butanol: hexane (50:50, v/v) under an alkaline medium, and then back extraction was done via acetic acid. Distinct derivatization techniques were employed for the sample preparation for GC-MS analysis, i.e., silylation and acylation. Derivatization approaches were optimized under different parameters, i.e., reaction temperature and reaction time. N-Methyl-N-(trimethylsilyl) trifluoroacetamide [MSTFA] was found to be the best sound derivatization reagent for the GC-MS analysis of glimepiride. Total ion current (TIC) mode was selected for the monitoring of ions of trimethylsilyl (TMS) derivative of glimepiride with an m/z ratio of 256. Distinct parameters like specificity, carryover, stability, precision, and accuracy were evaluated for validating the identification method. The GC-MS method is found to be linear and illustrated within the range 500 to 2500 ng/ml with the value of R2 (coefficient of determination) at 0.9924. The stability of the extracted and derivatized glimepiride was accessed with regard to processed/extracted sample conditions and autosampler conditions, respectively. Accuracy at each concentration level was within the + 15% of the nominal concentration. Precision (%) for the interday and intraday analysis was found to be in the respectable spectrum. Conclusion Henceforth, the proposed GC-MS method can be employed for the determination of glimepiride in biological matrices.

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The Indian travel & hospitality industry has exponentially developed and has the substantial potential of the amazing social and chronicled legacy, landscapes, and places of natural characteristic excellence spread over the nation. The Indian hospitality and travel industry helps the nation in generating revenue and plays a highly significant role in creating sources of foreign exchange. 39 million opportunities for new jobs were created in 2020. Moreover, the no. of jobs will be increased to 52.3 million in the travel industry of India by 2028. Covid19 has a severely deleterious influence on the educational system of the entire world. Thus, the entire educational platforms i.e. from kindergarten to the universities have been shut down due to the high density of population to avoid the spread of the deadly coronavirus. Propitiously, having gained the experience from the epidemic 2003 SARS, educationalists of the hospitality world are ceaselessly changing reaction activities to the obscure pandemic situation and applying their best to keep up the activity of the training framework. The study investigates 443 students of hospitality management which are in the final year of their graduation. Thus, this paper is an attempt to understand the perception of hotel management aspirants’, with special focus given to the housekeeping department.

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