The present study explores that the aqueous extract of the seeds of Nyctanthes arbor-tristis (aka night jasmine) is very efficient for the synthesis of stable AgNPs from aqueous solution of AgNO 3 . The extract acts as both reducing (from Ag ? to Ag 0 ) and capping agent in the aqueous phase. The constituents in extract are mainly biomolecules like carbohydrates and phenolic compounds, which are responsible for the preparation of stable AgNPs within 20 min of reaction time at 25°C using without any severe conditions. The synthesized silver nanoparticles were characterized with UV-Visible spectroscopy, FT-IR, XRD and SEM. UV-Vis spectroscopy analysis showed peak at 420 nm, which corresponds to the surface plasmon resonance of AgNPs. XRD results showed peaks at (111), (200), (220), which confirmed the presence of AgNPs with face-centered cubic structure. The uniform spherical nature of the AgNPs and size (between 50 and 80 nm) were further confirmed by SEM analysis.

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Objective:This study was designed to analyze the combinatorial chemotherapeutic effect of temozolomide (TMZ), the most common drug in glioblastoma treatment and a purified carbohydrate (Fr-II) from the edible mushroom Pleurotus florida, on human glioblastoma cell lines.Methods: Fr-II was purified by size-exclusion chromatography and characterised by different mass spectroscopy analysis. Human glioblastoma cells were treated with TMZ, Fr-II, and combination of TMZ and Fr-II. Cell cytotoxicity was measured by MTT assay, cell cycle phase distribution was determined by cell cycle analysis and followed by the relative p53 protein expression was analyzed by western blot analysis.Results: Chemical analysis of Fr-II confirmed the glycosidically linked two units of glucose with terminally attached mannitol with mass of 506 Da. Fr-II treatment exhibited cytotoxicity in both the cell lines in a dose-dependent manner with most effective dose at 200µg/ml. When Fr-II (200µg/ml) was combined with a dose range of TMZ it showed a more cellular cytotoxicity compared to the cytotoxicity of TMZ alone with most oppressive combinatorial dose at 400µM (TMZ)+200µg/ml (Fr-II). In compliance, with the above results, both cell lines showed a 10% increase in no. of cells (p<0.05) in G2/M phase indicating an arrest of cell cycle and increased p53 protein expression (p<0.05) at the combinatorial dose than TMZ alone at 400µM, but Fr-II alone didn't show any cell cycle arrest nor did it show increased p53 expression. Conclusion:Therefore it confirms that Fr-II synergizes with TMZ to significantly intensify its anti-proliferative properties, thereby emerging as an effective element for combinatorial treatment of glioblastoma.

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