Aims and Objectives:The aim of this study is to compare the esthetic improvement of white spot lesions (WSLs) treated by icon, sodium fluoride (NaF), and bioactive glass using VITA Easyshade® spectrophotometer.Methodology:Ninety intact human maxillary central incisors were collected and artificial WSLs were created on facial surface having dimensions of 4 mm × 4 mm by immersing in demineralized solution for 4 days, baseline comparisons were performed by measuring the color of the WSLs compared to the adjacent sound enamel. All samples were divided into three groups of 30 each. Three groups were Group 1: Treated by NaF, Group 2: Treated by the bioactive glass, and Group 3: ICON®-DMG America (Resin infiltration). After treatment, the specimens of all groups were stored in artificial saliva and reevaluated at 4 and 8 weeks after the beginning of the treatments with VITA Easyshade® spectrophotometer.Results:Statistical analysis was performed with One-way analysis of variance. Post hoc analysis with the Tukey's honest significant difference test was used to compare the data between the groups. Among three groups, resin infiltration has significant color change (ΔE) of infiltrated lesions when compared to other treatment groups (P < 0.001)Conclusion:Within the limitations of this study, it can be concluded resin infiltration (ICON®) can improve the esthetic characteristics of WSLs. Resin (ICON®) is a better treatment option when compared to bioactive glass and NaF as concerned to esthetics.