A study was conducted in two selected districts of arid region of Rajasthan i.e. Barmer and Bikaner. From each selected district, two tehsils were selected purposively. She and Chohtan tehsils from Barmer district and Kolayat and Lunkaransar tehsils from Bikaner district were selected on the basis of highest livestock population and two villages were selected randomly from each tehsil. Thus, total eight villages were selected and from each selected village, 15 respondents were selected randomly making the sample size of 120 livestock owners in present study. Data were collected from livestock owners with the help of semi-structured interview schedule, researcher's own observations were also recorded regarding the breeding practices followed by livestock owners. From the study it was found that majority (40.8%) of the livestock owners had high level of knowledge followed by medium (32.5%) and low (26.7%) level knowledge about breeding aspect of Organic Animal Husbandry practices. The sub-areas under different organic breeding practices in which the livestock owners had highest knowledge was promoted animals for natural service and knowledge of heat detection (mean score 1.00 for each) from breeding practices and the livestock owners had highest adoption about traditional methods for heat detection (adoption index 99.58). It was concluded that Livestock owners in Arid Region of Rajasthan mostly kept desi (local) breeds and preferred natural service for reproduction. These practices of farmers were well fitted with the standards of organic livestock farming related to breeding aspect.