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A series of biologically active Mannich bases with heteroaromatic ring system have been synthesized employing Mannich reaction of 2-amino-9 [{(1,3 dihydroxy propane-2yl) oxy}methyl] 6-9 dihydro-3H-purin-6-one ( potent drug) with biologically active sulphonamides and secondary amines. They were analyzed by elemental analysis and characterized by spectral studies-UV, IR, 1 H NMR, Powder X-ray diffraction and Scanning Electron Microscopy. The Mannich bases were screened for their antibacterial activity against various gram + and gram -bacteria. The results had shown that the Mannich bases are quiet active against pathogens under study at varying concentrations. The toxicity of synthesized Mannich bases was ascertained by LD 50 test.

Contrary to the typical treatment system, built wetland systems have become high-performance wastewater treatment technology in recent years. In India as well, this technology is becoming more important for reducing water pollution. A built wetland is essentially a tank that has been planted with plants tolerant of waterlogged conditions and filled with a substrate. In this method, wastewater is treated by plants through phytoremediation. A manmade wetland's plant species is crucial in maintaining the temperature needed for a variety of biological and physiological processes necessary for the efficient treatment of wastewater. Hydraulic retention time (HRT), plant type, and bed material make up a built wetland's key elements. Generally, gravels and sand are used as the bed media and the plant species used are grasses like typha grass, canna indica, para grass, etc. Environmental-related parameters that are taken into consideration are pH, COD, BOD, TSS, NH3-N, PO4, nitrate, and Fecal coliform count in one complete macrophyte life cycle. The present paper gives information about the different types of constructed wetlands, pollutants removal mechanism by microphytes, engineering design used, and application of implemented wetlands.

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