The examination of sperm concentration in the laboratory is the calculation of the number and motility using a microscope or using a device. There are still some clinicians who doubt the accuracy of the sperm count results using a semen analyzer rather than using the manual method. This study aims is to determine the differences of the sperm concentration examination between the manual method and the automatic method. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA "Restu Ibu" Sragen from June to August 2020. The object of this research is the examination of sperm concentration, using a manual method using a hemocytometer and an automatic method using the LensHooke ™ SQA X1 Pro. The results of statistical tests using the Mann Whitney methods show that the significance value (p) was 0.960, which means that there was no difference in the results of  the sperm concentration examination between the manual method and the automatic method. Result of this research shows that there is no weakness or significant difference if compared between manual and automatic methods.

According to World Health Organization data, 30-40% of infertility is caused by male factors. The morphology of normal spermatozoa is an indicator of male fertility, and it is known by manual or automatic sperm analysis. Lenshooke SQA X1 PRO automatic equipment comes along with the development of laboratory equipment automation technology. The working principle of this tool is by shining light on the object of examination, then the camera with high resolution, with the facility of an optical lens will take a picture of the object. The database recorded by the camera is analyzed by the algorithm. The research objective was to test the suitability of the Lenshooke SQA X1 PRO automatic tool with manual method as the Gold Standard. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA "Restu Ibu" Sragen from June to August 2020. The examination method used an automatic method with the Lenshooke SQA X1 PRO tool and a manual method with Papanicolaou staining. The results of the study, conformity test with WHO 2010 normal standards, automatic methods reached 94.4% compared to manual methods. The next statistical test was with standard mean, normal sperm morphology data had a significance of 0.001, abnormal sperm head data had a significance value of 0.956 and abnormal sperm tail data had a significance value of 0.339. The Lenshooke SQA XI PRO device based on automatic technology can be used in laboratory services for sperm analysis in addition to manual methods. Suggestions for using the Lenshooke XI PRO automatic tools are still accompanied by the manual method.