2019 – Coronavirus diseases caused by severe acute respiratory syndrome coronavirus 2. In Gujarat, COVID-19’s first two cases were confirmed in Rajkot and Surat on 19th March 2020. In India to prevent COVID-19 cases from being: “test, track and treat”: For testing, RT-PCR is the gold standard test or frontline testing for the Coronavirus. The most prominent cause of infection in health care workers due to they were directly exposed to unrecognized COVID-19 patients. To determine the Infection rate and also assess the clinical profile of COVID-19 infections in health care workers. Sample collected from 2081 health care workers by universal sampling method during the period from April 2020 to November 2020. Nasopharyngeal and Oro-pharyngeal swabs were collected at the collection center and tested at the Viral Research Diagnostic Laboratory (VRDL), by RT-PCR (Real-Time Polymerase Chain Reaction) Method. During the study period, there were 2081 suspected healthcare workers samples received from 70 Health care workers were positive. We observed 3.36 % Positivity rate, positivity was high in the age group of 20–30 years 64.2%, most common clinical presentation was a fever 82.8%, cough 47.1% and Sore throat 50% and 01 severely infected health care worker died during hospitalization. Health care workers were at a higher risk of COVID-19 infection. So, to protect health care workers routine screening of asymptomatic staff and early identification of staff with potential infection could help to lower the risk. COVID-19 mortality was lower in health care workers.

Introduction: Dengue has rapidly emerged as a vector - borne viral disease in recent years and also endemic in all continents. The agent of dengue, i.e., dengue viruses, are categorised under the genus Flavivirus, with the four dengue virus serotypes: designated as DENV-1, DENV-2, DENV-3 and DENV-4. These all four serotypes are in circulation either singly, or more than one at the same time. Aim: To study the epidemiological update of dengue with circulating serotype and co-infection in Saurashtra region, Gujarat, India. Materials and Methods: A cross-sectional study was conducted during January 2019 to December 2020 and total samples received were 12,563 which were clinically suspected dengue samples case. After receiving blood samples, serum was separated and proceeded for Dengue NS1Ag (nonstructural protein 1 antigen), and Dengue IgM Ab (Immunoglobulin M antibody). After serological confirmation, 151 samples from different geographical area were selected for Dengue specific Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR) for serotyping. results: Total 4069 (32%) had confirmed dengue positive by Enzyme Linked Immunosorbent Assay (ELISA). The ratio of male cases was higher than female, and in age group 21-35 year (47%). Seasonal trend showed a gradual increase in positivity from June with high peak in October. Circulation of all the four serotypes in area, higher monotypic infection by DENV-1 serotype (41%), followed by DENV-4, DENV-2 and DENV-3. Co-infection of different serotypes were also found. conclusion: The present study concluded that all four serotypes circulate with predominant being DENV-1 type and co-infection of different serotypes in the saurashtra region. Dengue mainly affected adult male population, and seasonal peak during monsoon and post-monsoon period.