SummaryPorcine circovirus-like virus P1, a novel single-stranded circular DNA virus, is a potential causative agent of post-weaning multisystemic wasting syndrome in swine.In this study, we detected P1 strains in serum samples from cattle, goats and rabbits suffering different clinical symptoms, in Jiangsu Province, China. We sequenced the whole genomes of six P1 strains, which showed 99.4%-100% nucleotide sequence similarity and were closely related to other porcine-origin P1 isolates. The sequences of six non-porcine-origin P1 viruses were most closely phylogenetically related to other porcine-origin P1 sequences. Therefore, the P1 virus has a wide host range in China. , 2012). In this study, we report the detection of P1 virus in cattle, goats and rabbits in China. | MATERIALS AND METHODSIn May 2016-January 2017, a surveillance of P1 virus was conducted in Jiangsu Province, China. A total of 300 serum samples, including 120 samples from four cattle farms, 120 samples from three goat farms, and 60 samples from two rabbit farms, were collected and tested for P1 viral DNA with PCR and primers specific for the complete genomic sequence of the P1 virus, as described previously (Wen, Jiao, Zhang, Li et al., 2017). | RESULTS AND DISCUSSIONViral DNA was detected in 5% (0/30, 4/30, 2/30 and 0/30 on the four cattle farms), 7.5% (0/40, 4/40 and 5/40 on the three goat farms) and 10% (0/12 and 6/48 on the two rabbit farms) of the samples. The sequences were submitted to GenBank under accession numbers KY462783 and KY462784 (cattle), KY462785 and KY462786 (rabbit), and KY462787 and KY462788 (goat). The calves and immature rabbits from which P1 was obtained showed unexplained weight loss, whereas the goats showed mild respiratory signs.A complete genomic sequence analysis performed with Clustal-W indicated that the six P1 sequences shared 99.4%-100% overall

Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33 QKVY 36 within Cap protein. Sequence alignment indicated that 33 QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.