Nanotechnology is gaining prime importance in the present era due to modeling of metals in nanoparticles (NPs) by biological methods, but nowadays, researchers are designing the exact mode of action of nanomaterials on plants. NPs are fabricated by different physical and chemical methods, but biological methods are preferred due to their simplicity and non-toxic nature. The current development of biomimetic NP synthesis is a more reliable, economically favorable and eco-friendly method for the treatment of different diseases. NPs fabricated by traditional methods have shown a lot of demerits, so the green route to the formation of metallic NPs is advantageous compared to the use of microbes. Secondary metabolites in the plant have active chemical constituents which can act as capping and reducing agents, thereby enhancing the rate of reduction and stabilizations of NPs. In this review, a major focus is given to biogenic silver NPs’ mechanism of action toward cancer and microbes.