The presence of hydrogen sulfide in natural gas will reduce the calorific value of natural gas, so hydrogen sulfide in natural gas needs to be removed. Titanosilicalite zeolite is a stable porous material with good catalytic effect and has attracted much attention due to its excellent catalytic oxidation performance in the system composed of it and hydrogen peroxide. Therefore, in this paper, the removal of hydrogen sulfide in natural gas was studied by using titanosilicalite zeolite as catalyst and hydrogen peroxide as oxidant, and the mixture of hydrogen sulfide and nitrogen gas was used as the simulated gas of natural gas. The experimental results show that the titanosilicite zeolite has a good catalytic effect on hydrogen sulfide. The influence of the concentration of hydrogen peroxide, reaction temperature and pH of the initial solution in the process of removing hydrogen sulfide by titanosilicalite zeolite was studied.