Background. The pursuit of health and longevity is the eternal theme of humanity. Guangxi has a remarkable phenomenon of longevity in long-lived regions and ranks with the highest number of longevity villages in China, thus providing a natural advantage for health longevity research. Methods. In this study, we selected 117 natives of a longevity area in Guangxi, covering a large age range (38-118 years old) as subjects to measure peripheral leukocyte telomere length (LTL). Nineteen physical examination indicators and two inflammatory factor levels were measured. Results. Pearson’s analysis revealed a significant negative correlation between age and LTL ( r = − 0.3694 , p = 0.003 ), as well as alanine aminotransferase, albumin, total bilirubin, direct bilirubin, γ-glutamyltransferase, triglycerides, Interleukin-10, and tumor necrosis factor type-α. Systolic blood pressure and blood urea nitrogen were positively correlated with age. In addition, LTL decreased in people aged 38–89 years, and an upward trend was observed in people aged older than 90 years. Conclusions. Longevity individuals have characteristics, such as longer LTL, good hepatic function, and lower triglycerides and inflammation levels.