The crane fly Limonia phragmitidis belongs to family Limoniidae. The mitogenome of L. phragmitidis was sequenced, the new representative of the mitogenome of the family Limoniidae. The nearly complete mitogenome is 16,020 bp totally, consisting of 13 protein-coding genes, 2 rRNAs, and 22 transfer RNAs. All genes have similar locations and strands with that of other published species of Limoniidae. The nucleotide composition biases toward A and T, which together made up 75.5％ of the entirety. Bayesian inference analysis strongly supported the monophyly of Tipuloidea. It suggested that the phylogenetic relationship within Tipuloidea, Pediciidae þ (Limoniidae þ (Tipulidae þ Cylindrotomidae)).

The mitochondrial genome of Trypetoptera punctulate Scopoli, 1763, the first representative of Sciomyzidae, was sequenced and annotated. And we used this new nucleotide information under Maximum Likelihood analyses to explore the superfamilies relationships of Acalyptratae. The nucleotide composition of Trypetoptera punctulata mitochondrial genome was 34.6% of A, 33.9% of T, 17.6% of C, 13.9% of G, 68.5% of A þ T content. The start codon ATG was shared with COX2, COX3, CYTB and ND4; the start codon ATA was shared with ATP6, ND5 and ND6; the ATP8 and ND1 started with codon ATT; the COX1 and ND3 started with codon ATC, and the ND2 and ND6 started with codon TTG. The conservative stop codon TAA was shared with ATP6,

The dance fly Oreogeton sp. belongs to the subfamily Oreogetoninae of Empididae. The mitogenome of Oreogeton sp. (GenBank accession number: MK639348) was sequenced, the first representative of the mitogenome of the subfamily. The nearly complete mitogenome is 15,718 bp totally, consisting of 13 protein-coding genes, two rRNAs, and 22 transfer RNAs. All genes have the similar locations and strands with that of other published species of Empididae. The nucleotide composition biases toward A and T, which together made up 77.2％of the entirety. Bayesian inference analysis strongly supported the monophyly of Empidoidea, Empididae, and Dolichopodidae. This result also suggested that Oreogetoninae was assigned to the sister group to the clade that consists of Trichopezinae and Clinocerinae, and then Empidinae is the sister to the clade that contains Oreogetoninae, Trichopezinae, and Clinocerinae.

Twelve species of the subgenus Vestiplex (Diptera, Tipulidae) were previously known to occur in Tibet (= Xizang), China. Here, six species are described and illustrated as new to science: Tipula (Vestiplex) bucera sp. nov., Tipula (Vestiplex) magatama sp. nov., Tipula (Vestiplex) motuoensis sp. nov., Tipula (Vestiplex) nayogabuensis sp. nov., Tipula (Vestiplex) platyphylla sp. nov. and Tipula (Vestiplex) uncinella sp. nov. The following three species are redescribed: Tipula (Vestiplex) himalayensis Brunetti, 1911, Tipula (Vestiplex) nigroapicalis Brunetti, 1911 and Tipula (Vestiplex) zayulensis Alexander, 1963. A key to the species of Tipula (Vestiplex) from Tibet is presented.