No abstract

This study examined the e¡ects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for10,30,60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no di¡erences in the fertilization rates and hatching rates between sperm cryopreserved for o60 days and fresh sperm (98.8 AE 0.8%, 96.4 AE 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 AE 3.0% to 7.0 AE 1.9%) and hatching rates (79.4 AE 7.2% to 3.3 AE 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality signi¢cantly (Po0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations.We suggest that 15% DMSO may be an e¡ective cryoprotectant for long-term sperm cryopreservation of red seabream. Aquaculture Research, 2010, 41, e89^e95 Changes of sperm quality after cryopreservation Q H Liu et al. r 2010 The Authors Journal Compilation r 2010 Blackwell Publishing Ltd, Aquaculture Research, 41, e89^e95 e91Changes of sperm quality after cryopreservation Q H Liu et al.