BackgroundIt has been reported that lncRNA DSCAM-AS1 plays an oncogenic role in breast cancer. In the present study we explored the role of DSCAM-AS1 in colorectal adenocarcinoma (CRA).Methods: Gene expression was analyzed by qPCR and Western blot. Overexpression experiments were performed to analyze gene interactions. Transwell assays were performed to analyze cell invasion and migration. Methylation-specific PCR (MSP) was performed to analyze DNA methylation.ResultsIt was observed that DSCAM-AS1 was upregulated in the primary tumor tissues than in paired non-tumor tissues (within 2 cm around tumors) and was further increased with tumor metastasis. miR-216b was downregulated in primary tumor and further downregulated with tumor metastasis. miR-216b was inversely correlated with DSCAM-AS1 in tumor tissues, but not in non-tumor tissues. In cells of CRA cell lines, DSCAM-AS1 overexpression resulted in the downregulation of miR-216b, while miR-216b overexpression did not significantly affect DSCAM-AS1. DSCAM-AS1 overexpression did not significantly affect cancer cell proliferation but promoted cell migration and invasion. miR-216b inhibited cancer cell migration and invasion and significantly reduced the effects of DSCAM-AS1 overexpression. Methylation-specific PCR showed that DSCAM-AS1 overexpression promoted the methylation of miR-216b gene.ConclusionDSCAM-AS1 may downregulate miR-216b to promote the migration and invasion of CRA cells.

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The control system for the fixed-frequency boost converter does not consider the situation from the input voltage and the unknown load, which makes the system vulnerable to external disturbances, resulting in slow system response and large output voltage error. The state space equation of the boost converter is established by the state space averaging method, and the adaptive control rate of the load resistance and the input voltage is designed by the adaptive control, which effectively reduces the influence of the disturbance on the system output. The sliding mode surface adopts the integral terminal sliding mode surface, and the isokinetic approach law control is used to effectively reduce the influence of chattering and improve the dynamic response speed and robustness of the system. The simulation experiment proves the validity and reliability of the integral terminal adaptive sliding mode control.

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