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A colorimetric and near-infrared fluorescent probe for thiophenols was developed. This new probe features remarkable large Stokes shift, and shows a rapid, highly selective and sensitive detection process for thiophenols with obvious colorimetric and near-infrared fluorescent turnon responses. The detection limit of this probe for thiophenol was found to be 70 nM. In addition, this probe shows great potential for in vitro and in vivo detection of thiophenols, which was exampled by the successful applications of this probe for quantitative detection of thiophenol in real water samples and fluorescent imaging of thiophenol in living cells and zebrafish.

A new ratiometric fluorescent probe was reported for the rapid detection of toxic thiophenols.