Known as “living fossils”, large old trees play an important role in ecology, landscape, and culture and are an important part of ecosystems and human settlements. The aim of this paper is to provide suggestions for the protection of urban large old tree resources and the selection of large old tree backup resources. First, we conducted a statistical analysis on the composition, distribution, and important values of large old tree species in Beijing and used Downtrend correspondence analysis (DCA) to analyze the composition of different types of habitat tree species. Second, we created a 3 km × 3 km grid within the administrative scope of Beijing, extracted the number of large old trees and tree species richness in the grid, and used geographic detectors to determine the driving factors of the spatial distribution of large old trees, as well as tree species richness differences and their interactions. A total of 40,590 large old trees in Beijing were found, belonging to 72 species, 52 genera, and 29 families. Platycladus orientalis (L.) Franco was the dominant tree species, with an importance value of 0.51. Among the different habitats, the large old trees were found in parks and temples, and the greatest tree species number of old trees were found in communities and the countryside; meanwhile, microgreen spaces had the lowest number of trees and tree species. The distribution of large old trees and tree species was mainly concentrated in the center of the city and the northwest. The distribution pattern of large old tree resources in Beijing is affected by the interaction of various factors. Social factors were the dominant in the distribution of large old tree resources in Beijing. The spatial distribution of large old trees was mainly affected by the scenic resort and historic site (SRHS), and the SRHS and gross domestic product (GDP) level were the most important factors influencing the richness of large old tree species. In addition, the functional value and characteristics of tree species determined the distribution of large old tree habitats. Therefore, the protection of large old tree resources requires developing scientific management and planning by managers, increasing investment in management and protection, and strengthening ecological culture publicity.
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