Recent studies have reported that long noncoding RNAs (lncRNAs) play critical roles in carcinogenesis and progression. LncRNA-LET, a recently identified lncRNA, has been shown to be a tumor suppressor in hepatocellular carcinoma. However, the expression and functional of lncRNA-LET in other type of cancers remain largely unknown. In this study, we found that lncRNA-LET was significantly downregulated in nasopharyngeal carcinoma (NPC) tissues compared with corresponding normal tissues. Decreased LET expression is significantly correlated with advanced clinical stage, larger tumor size, increased lymph node tumor burden, and poor survival of NPC patients. Gain- and loss-of-function experiments demonstrated that enhanced LET expression inhibited NPC cells proliferation and induced cell apoptosis. By contrast, the knockdown of LET promoted NPC cells proliferation and inhibited cell apoptosis. Importantly, we found lncRNA-LET is transcriptional repressed by EZH2-mediated H3K27 histone methylation on the LET promoter. The expressions of EZH2 and lncRNA-LET are significantly inversely correlated in NPC tissues. Collectively, these findings indicate a pivotal role for lncRNA-LET in NPC cell proliferation and apoptosis, and reveal an epigenetic mechanism for lncRNA-LET dysregulation.

To explore the effect of evidence-based nursing on the application of ultrasonic emulsification operation for cataract and the postoperative rehabilitation indicators. 110 cataract patients were included. All of them received ultrasonic emulsification operation for cataract and were divided into the control group(n=55) and the observation group(n=55) by random number method. The control group received routine nursing. The visual acuity, intraocular pressure, quality of life and adverse reactions of patients in both groups after discharge were compared and evaluated. The clinical effective rate(92.73%), postoperative visual acuity(0.68 ± 0.05) and SF-36 score(82.37 ± 4.13) in the observation group were higher than those in the control group(76.36%), (0.55 ± 0.02) and(74.06 ± 3.42), Conclusion: Evidence-based nursing can effectively improve the curative effect and postoperative vision, and improve the quality of life. The intraocular pressure in the observation group(9.95 ± 2.46) mmHg and the incidence of adverse reactions(29.09%) were lower than those in the control group(11.03 ± 3.11) mmHg and 10.91%(P<0.05). It is of distinct effect to carry out evidence-based nursing for the cataract patients receiving emulsification therapy. It effectively improves the effect of surgical treatment, reduce the occurrence of adverse reactions, so as to improve the quality of life and promote rapid physical recovery, maintaining clinical application value.