Background: Ginger oil has long been used in the food and pharmaceutical industries to cure vomiting, abdominal pain, dyspepsia, diarrhea... The subject aims to extract, determine chemical composition and antibacterial activity of ginger oil in Thua Thien Hue. Materials and method: Materials: Rhizomes of Zingiber oficinalis. Method: hydrodistillation method, gas chromatography and mass spectrometry analysis, and diffusion method on agar-agar disk. Results: Extraction process: Materials was milled to semi-fine powder, extracted in 3 hours, solvent-materials ratio was 3:1, with material within 10 days of harvesting. GC-MS analysis revealed 16 constituents in the ginger oil, in which the main component was Alpha-zingerberene. Ginger oil possessed antibacterial activity against Staphylococcus aureus and Bacillus subtilis with the same MIC values of 43.8 mg/ml. Conclusion: The results were contributed to determine extraction process, chemical composition and antibacterial activity of ginger oil in Thua Thien Hue. Key words: Ginger oil, antibacterial, extraction, Alpha-zingerberene, Zingiber officinale