Objective: fingerprints are permanent and an individual physical characteristic, and are considered an authentic proof of identification. The aim of the present study was to investigate whether fingerprints have any significant relationship with gender and blood group. Materials and Methods: the fingertip patterns of 400 Saudi subjects were recorded using ink and paper, and identified by magnification lens, and the blood group was determined by the agglutination method. After recording the blood group and fingerprints of the subjects of both genders, their correlation was analyzed by Chisquared test. Results most subjects (49.25%) belonged to blood group O, followed by A, B and AB, of which 92% were Rhesus (Rh) positive; the relationship between gender and blood group was statistically significant. Regarding the fingertip patterns among the genders and ABO and Rh factor blood groups, loopsweremore frequent, followed by whorls and arches. Regardless of the Rh factor, the fingerprint pattern in blood groups A and AB was statistically significant, but it was not significant in the B and O groups. The incidence of loops was higher on the middle and little fingers, as well as on the thumb, in blood groups A, B and O, and this relationship was statistically significant. Conclusions: the present study reveals that the fingerprint pattern is not associated.