A physical map of the M. neustria nuclear polyidrosis virus (ManeNPV) genome was constructed, the complete order of BamHJ, Kpnl and Pstl restriction enzyme sites was determined t a polyhedrin gene was localized on the map. The viral DNA size was calculated to be about 139 kbp. Restriction endonuclease profiles of the DNA of ManeNPV plaques isolate propagated in A. pernyi cells demonstrated ^persistent heterogeneity*, submolar bands were shown to appear in a digestion pattern of DNA of the first passage virus. These bands were proved to be due to the DNA molecules presence in the non-homogeneous virus DNA pool, their chains having been shown to carry a putative break in a definite site. Such a *break site» was localized on the physical, map of ManeNPV genome. The Baculoviridae contain double-stranded circular DNA viruses infecting a lot of species belonging mainly to the Lepidoptera> Diptera> and Hymenoptera orders. Baculoviruses with their large genomes and complex reproduction cycle accompanied by the cas cade gene expression regulation have become an interesting and well-made topic of molecular biology studies. The interest to the representatives of this family is also caused by three aspects of their practical use. First of them is an application of the baculoviruses as vectors for gene therapy, the problem being investigated during last decade [1, 2]. Two other aspects are more traditional: baculoviruses are considered as viral insecticides for pest insect control and are widely used as vectors for recombinant protein synthesis in both in vivo and in vitro cul tivated insect cells [3 J. M. neustria {Mane) larvae (Lepidoptera, Lasiocampidae) is a well known garden fruit pest insect, especially on apple trees. However, outbreaks of the pest mass reproduction occur often also in woods (first of all, in oak woods) of the forest-steppe zone.

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Fast and simple spectrophotometric method for quantitative determination of mometasone furoate in a single dose (single actuation) of its nasal spray was developed and validated. This method is based on the spectrophotometric analysis of turbid solution of a single spray in 20 mL isopropanol at 220–310 nm. We further show applicability of this method for analysis of large number of single sprays for the dosing homogeneity. It is based on a new approach to the development of spectrophotometric quantitative determination methods for drug products and possibly other objects, allowing measurements to be carried out on sufficiently turbid test solutions. The proposed approach is not a variant of the derivative spectrophotometry and can replace the methods of derivative spectrophotometry in cases where: derivative of analyte spectrum is not intense enough; derivative of the spectrum of the matrix (turbidity and remaining components of the product) is significant when compared with the derivative of the analyte.

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