Hibiscus is a genus of a gorgeous flowering potted plant. Hibisceae tribe is one of the ornamental plants that are most commonly available and it belonging to Malvaceae family is Hibiscus rosa-sinensis. The purpose of this work is to evaluate the in-vitro anti-athritic effect of Hibiscus rosa sinensis at various levels of concentration. Here the percentage of membrane stabilization was carried out at various concentrations for ethanolic extract and Diclofenac sodium. Hibiscus extract was found to have maximum membrane stabilization of 94.97% at 500μg/ml. The highest percentage inhibition of denaturation of protein and membrane stabilization for these plant extracts were found to be 89.45% and 91.09% at a dose of 500μg/ml. Our research studies support the isolation and the use of active phytochemicals of hibiscus in the treatment of inflammation and arthritis.