Hemoglobin iron-containing metalloprotein expressed in red for all vertebrate blood cells (erythrocytes), as well as some invertebrate tissues. A healthy person has 12 to 20 grams of hemoglobin in every 100 milliliters of blood. Hematocrit is a blood test measuring the percentage concentration of red blood cells (RBC) in the blood. The measurement will depend on the number and size of cells in red bloods. It is part of the complete results of a person's blood count, along with hemoglobin concentration, white blood cell count, and platelet count. The volume of packed cells (PCV) is a measure of the proportion of blood formed by cells. The value is expressed as one percentage or fraction of blood cells. For example, a 40 per cent PCV means that in 100 milliliters of blood there are 40 milliliters of cells. This study is to compare the Hb and PCV values derived from direct manual and automated methods. Nowadays PCV values are estimated through automated methods. If there are any electrical supply problems, low budget labs, unavailability of the wintrobe's tube, in a practical college set up we have sahli's haemoglobinometer , with this we can be able to calculate the PCV valve. But our results show that the automated PCV values are higher than the manual calculated PCV values and the automated methods are more accurate to follow. Also we found that the manual calculated PCV method is not a better alternative for Automated PCV method.