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Introduction: Puberty in adolescents is always accompanied with many psychological changes and they require education during this period of life. The need for education in adolescent females with vision loss is more sensitive.Objective: This study aimed at investigating health education needs of puberty in blind adolescent females at specialized centers in Tehran in the academic year of 2012 to 2013. Materials and Methods:The present research was a descriptive study. One hundred blind females between 9 to 19 years old were studied based on the use of simple sampling during the academic year of 2012 to 2013. The data collection tool was a self-made questionnaire including 30 questions, which was used by samples after the confirmation of validity and reliability. For data analysis, the SPSS software version 16 was used. Results:The rate of desirable awareness regarding physical and psychological health was 12% and 28%, respectively, and the most important source of obtaining information was the participants' mothers with a frequency of 42%. Discussion and Conclusion:Puberty education is a necessity in order to increase awareness of blind girls in relation to physical and psychological health. Since these adolescents' mothers were the most important source of obtaining information, the researchers could achieve positive outcomes with education based on the adolescents needs.