The infection rate of Hepatitis C virus in chronic hemodialysis (HD) patients is comparitively higher than the normal population. Hepatocellular carcinoma and cirrhosis are the major complications that have been frequently reported among the patients with HCV infection who were on hemodialysis. Hence, continuous monitoring of HCV infection in hemodialysis settings at health care centres may aim towards the assessment and prevention of device induced infection. Hence this current study was aimed to detect the HCV RNA using PCR among hemodialysis patients attending JSS, a tertiary care centre situated in Mysuru, South India.