Over the last 30 years, Burn-out Syndrome has been mainly matched to psychological discomfort among the staff in Oncology Departments, therefore, it has been considered as the unique issue to be diagnosed, treated, and prevented. Our study is aimed to evaluate the relation between psychological states and traits with different Male/Female (M/F) ratio was 18/54. Median age was 37 years old (range from 20 years old to 62 years old). We did not show a significant correlation between depression and operators' age, years of work, professional role, and relational decline, while a statistically significant association was observed between depression and professional ineffectiveness (p=0.042), disillusion (p=0.0003), and psychophysical exhaustion (p = 0.00001). According to STAXI 2, 15% of the personnel had a high expression of aggressiveness while 10% was over-controlled.Aggressiveness was statistically related to depression (p = 0.001), disillusion (p = 0.009), and psychophysical exhaustion (p = 0.012). This study showed that aggressiveness and depression play a crucial role in psychological discomfort in an Oncology Department operators. Therefore, they should be taken into account together with burn-out when performing screening procedures for the psychological discomfort in the same setting.