Organic material is widely used for propagation of orchids, namely coconut water, extracts of tomatoes, bananas, bean sprouts and potatoes which can be added to certain media as a provider of nutrients, amino acid minerals, and growth regulators. This study aims to determine the effect of the concentration of organic matter in coconut water and banana extract on MS media on the regeneration of PLB (protocorn like bodies) Dendrobiumgatton sunray. The study was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Bengkulu University, from September to December 2019. This study used a Complete Randomized Block Design (RCBD) with two factors. The first factor was the concentration of coconut water with 3 levels namely: A0 = 0 (Control), A1 = 75 ml.L -1 , A2 = 150 ml.L -1 , and the second factor was the concentration of banana extract with 5 levels namely: B0 = 0 (Control), B1 = 75 g.L -1 , B2 = 150 g.L -1 . The results showed that coconut water did not affect all variables, however the banana extract had a significant effect on all variables except for the number of shoots. There was an interaction between the two treatments, namely the variable plantlet weight. Orthogonal polynomial analysis showed that there was an interaction between the two treatments, where coconut water and banana extract had a good response on the plantlet growth when given together as much as 75 ml.L -1 (A1) and 75 g.L -1 (B1). The concentration of both treatment was lower than it was given individually.

Penyusunan modul “Pemberdayaan Masyarakat Sekitar Hutan Berbasis Konservasi dan Budidaya Kopi Ramah Lingkungan” ini dimaksudkan untuk memberikan panduan kepada para penerima manfaat dalam menjalankan upaya pelestarian hutan dan penerapan budidaya kopi yang ramah lingkungan (berkelanjutan). Penyusun mengucapkan terima kasih yang sebesar-besarnya kepada ICCTF (Indonesia Climate Change Trust Fund) dan Yayasan Leuser Internasional (YLI) yang telah mendukung penulisan dan perbanyakan modul pelatihan ini.

Micropropagation is an alternative method for producing pathogenic contamination-free shallots seedlings. The aim of this research was to determine the optimum balance of auxin-cytokinin and sucrose concentration in stimulating the plantlet regeneration on shallot. The research was conducted from March to August 2019. In this study, the auxin and cytokinin treatment (1-5 ppm) were used in the format of a randomized complete block design in three replications. The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g.L -1 agar powder and were adjusted at pH 5.8 before autoclaving. The multiple shootlet (10 weeks of cultured) were then subcultured for bulblet formation in MS medium supplemeted with BAP dan succrose (3-9%). The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g L -1 agar powder and were adjusted at pH 5.8 before autoclaving. The results showed that the use of ½ MS media could increase the efficiency of using nutrient solution of tissue culture media. Micro shoot formation can be initiated on ½ MS medium with the addition of 1 ppm BAP (1.36 shoots / explants). This media also produced the best response for the number of micro roots (5.63 roots / explants), and the number of micro leaves (3.91 leaves). However, the difference in composition of nutrient in culture media was not able to accelerate the time of growth of shoots, roots and leaves. Further subculture in ½ strength of MS medium containing of BAP and sucrose 6% increased shoot, root and bulblet formation (13.3 shoots, 41.3 roots and 10.7 bulblets).