The purpose. To find out biological impact of various concentration of nano-materials in an organism of animals and to determine perspectives of their use in animal husbandry. Methods. Physiological, biochemical, nano-technological with the use of biostatistical analysis. Results. Researchers are carried out on determining physiological and biochemical mechanisms of action of nano-aqua-citrates of microelements in an organism of cattle, pig, rabbit and bee during different periods of ontogenetic development and productive use. Effect is studied of these joints on the content in tissues and liquids of macro-and microelements, formation of immunobiological reactivity of an organism, state of anti-oxidant, detoxification, reproductive and immune systems, growth of calves, pigs and rabbits, as well as role of citrates in treatment and prophylaxis of microelementosis at animals. Functional changes of separate systems and organs of an animal of different species are determined at action of nano-materials on the basis of biotic elements and synthetic polymers. Series of biological effects with activation of living functions and biochemical processes in an organism of animals is revealed. Expediency of use of functional nano-materials as activators of exchange processes and efficient means of target delivery and magnification of therapeutic action of medical products is proved. Conclusions. Biological effect of different concentration of nano-materials in an organism of laboratory and productive animals is found out; their stimulating effect on metabolic processes in physiological doses is shown. It is established that citrates of microelements are biologically active and safe-health, and their application raises grow power and productivity of animals. Expediency of use of nanomaterials on the basis of citrates of bio-elements in animal husbandry is proved.