Contamination with harmful chemical substances, including organic compounds of the BTEX and PAH groups, constitutes one of the major threats to the functioning of soil habitat. Excessive contents of the above substances can exert adverse effects on soil organisms, reduce biodiversity, and thus deteriorate soil quality. The threat to soil ecosystems within areas particularly exposed to contamination with accumulating chemical compounds was assessed using the Ecological Risk Assessment (ERA) with a multi-stage Triad (triage rapid initial assessment) procedure (taking into account the different lines of evidence). The article presents the results of chemical and ecotoxicological study of soils sampled at sites affected by contamination from petrochemical industry. The study results provided foundations for developing the site specific ERA framework for the area examined.
A collaborative study was conducted in 14 laboratories to evaluate the performance of the hydrophobic grid membrane filter method against the official final action method, 46.016, for enumeration of colif orms in foods. Twelve artificially contaminated samples of nonfat dry milk and canned custard were analyzed by each laboratory. The random errors (Sr2) associated with the hydrophobic grid membrane filter method were significantly lower than those of the official method, and the counts obtained by the new method fell within the 95% confidence interval of the reference method. The colif orm counts obtained by tiie hydrophobic grid membrane filter method did not differ significantly from those obtained by the reference method as measured by a 3-way analysis of variance. The new method has been adopted official first action.
Twenty laboratories participated in a collaborative study to validate a 2-day hydrophobic grid membrane filter method using YM-11 agar for enumeration of yeast and mold in foods. Six naturally contaminated food products were included in the study: garlic powder, raw ground beef, walnuts, flour/meal, orange juice, and yogurt. The test method produced significantly higher results than the 5-day pour plate reference method for orange juice and significantly lower, though numerically similar, results for walnuts and yogurt. Differences between the test and reference methods were not significant for garlic powder, raw ground beef, or flour/meal. Repeatability and reproducibility were similar for both the test and reference methods in all cases. The hydrophobic grid membrane filter method for enumeration of yeast and mold in foods has been adopted by AOAC INTERNATIONAL.
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