tubes, pipet 2 ml. of the standard solution and, for the blank, pipet 2 ml. of 50% v./v. of aqueous ethanol. All tubes are shaken on a mechanical shaker for about 2 minutes, then centrifuged for 30 seconds at about 1500 r.p.m. The aqueous layer is aspirated off, and the absorbance is measured at 620 µ against the reagent blank.
RESULTS AND DISCUSSION
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