Synopsis The protocol described here has been developed to measure the stability of UV-B filters; a modified version is recommended for UV-A filters. It should be considered as a tool to predict the effectiveness remaining after exposure to UV-A and UV-B light. It is a simple and reliable in vitro model simulating conditions of actual use. The results show that each filter requires an appropriate choice and fine tuning of reproducible analytical conditions. While absolute values are directly influenced by uncertainties in irradiance (dosimetry), comparative measurements with respect to a known standard are very reliable.

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The roots of Pimpinella saxifraga and P. major (umbel-

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A leaf-differentiating callus culture and a root organ culture of Pimpinella anisum have been established in liquid nutrition medium. Their behaviour in accumulation of phenylpropanoids was shown to be different from that of the entire plant. This makes them, together with their special growth behaviour, suitable for labelling experiments with the biosynthesis of pseudoisoeugenols.