The purpose of food packing in modified atmosphere is to extend its sustainability by preventing both biochemical processes and growth of spoilage bacteria. Gases or their mixtures which are mostly used in the modified atmosphere food packing technology are carbon-dioxide (CO2), oxygen (O2) and nitrogen (N2). The aim of our research was to examine the influence of packaging in modified atmosphere and vacuum on the total volatile basic nitrogen (TVB-N) content and pH in muscle of rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio), as well as to determine the most suitable gas mixtures for packing of these freshwater species. Three sample groups of trout and carp cuts were investigated. The first two groups were packaged in modified atmosphere with different gas ratios: 60%CO2+40%N2 (I group) and 40%CO2+60%N2 (II group), whereas the samples from third, control group, (III group) were vacuum packaged. During trials samples were stored in refrigerator at +3?C. Determination of TVB-N and pH was performed on 1st, 7th and 14th day of storage. The obtained results indicate that the investigated mixtures of gases and vacuum as well had a significant influence on the values of TVB-N in trout and carp cuts samples. The lowest increase in TVB-N was established in trout and carp cuts samples from the group I, whereas the highest increase was established in samples from group III. Statistical significant difference (p < 0,001) between the average values of TVB-N for trout (I group: 18,17 ? 0,93; II group: 20,90 ? 0,81 and III group: 36,18 ? 2,65 mg N/100 g ) and carp cuts (I group: 26,74 ? 1,48; II group: 30,02 ? 0,31 and III group: 35,10 ? 1,75 mg N/100 g) was established on 14th day. The lowest pH value was established in samples packaged in modified atmosphere with 60% CO2 +40% N2 (I group). On 14th day of testing the obtained value was 6,15 ? 0,09 for trout and 5,94 ? 1,11 for carp samples. Increase in pH value in trout samples packed in vacuum was established during the whole period of investigation (p < 0,001), while in carp cuts samples packaged in vacuum the increase in pH value (p < 0,05) was established up to 7th day of testing. Based on the obtained results it can be concluded that gas mixture consisting of 60% CO2 and 40% N2 was the most suitable for packaging of fresh trout and carp cuts in terms of selected chemical parameters, such as TVB-N and pH. [Projekat Ministarstva nauke Republike Srbije, br. TR 31011 i br. TR 31075]
The hares assayed (n=84) were divided into five age groups: 3-6; 12; 12-24; 24-36 and 36 + months. Between all sampling regions (11) significant differences of Cd levels were found in kidney and liver (p value, p=0.001 and 0.007, respectively) . Significant statistical differences (p=0.001) are registered between Cd content in kidney and liver of hares among all represented age groups. Looking at all investigated hare samples, moderately higher concentrations of Zn were found in liver (median value: 25.4 mg/kg w.w) compared to those in kidney (21.4 mg/kg). These differences were statistically significant (p=0.001). Zinc concentrations in liver, between all age groups, did not differ significantly (p=0.512) but in kidney these differences were statistically significant (p=0,001). Significant differences between Zn concentrations in liver in comparison to kidney (pairwise differences) were found within every single age group with exception of the oldest (36+ ) . Strong statistically significant correlations (Ps- Pearson's correlation ) between Cd concentrations in kidney and liver were registered in three groups older than 12 months (Ps=0.81, p=0.001; 0.78, p=0.001; and 0.79, p=0.001, respectively). Negative correlation between Zn and Cd concentrations were found in liver samples within the age group of 12 months (Ps= -0,67, p=0.004)
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