The current study was aimed to evaluate the wound healing activity of ethyl acetate extract of fruit peels of Citrus aurantium. An ethyl acetate extract of fruit peel of Citrus aurantium was loaded into an ointment base with three different concentrations (0.25% w/w, 0.5% w/w and 1%w/w) and tested for wound healing activity using excision wound model by measuring the healing area and dermal toxicity. Wound healing activity and histopathology studies confirmed that all the tested three concentrations of extract accelerate the wound healing process by decreasing the surface area of the wound and increases the muscle tensile strength. Out of tested ointments, 5% w/w concentration showed comparative activity with the standard. Hence, natural Citrus aurantium peel-based ointment is an alternative ointment in wound healing.