Image segmentation is a known problem in the fi eld of image processing. A great number of methods based on different approaches to this issue was created. One of these approaches utilizes the fi ndings of the graph theory. METHODS: Our work focuses on segmentation using shortest paths in a graph. Specifi cally, we deal with methods of "Intelligent Scissors," which use Dijkstra's algorithm to fi nd the shortest paths. RESULTS: We created a new software in Microsoft Visual Studio 2013 integrated development environment Visual C++ in the language C++/CLI. We created a format application with a graphical users development environment for system Windows, with using the platform .Net (version 4.5). The program was used for handling and processing the original medical data. CONCLUSION: The major disadvantage of the method of "Intelligent Scissors" is the computational time length of Dijkstra's algorithm. However, after the implementation of a more effi cient priority queue, this problem could be alleviated. The main advantage of this method we see in training that enables to adapt to a particular kind of edge, which we need to segment. The user involvement has a signifi cant infl uence on the process of segmentation, which enormously aids to achieve high-quality results (Fig. 7, Ref. 13). Text in PDF www.elis.sk.