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Sugar beet (Beta vulgaris L. subsp. vulgaris var. altissima) is a biennial, sugar-producing tuber crop grown in different parts of the world. Diseased sugar beets with symptoms of little leaf were observed during 2008 in Tamil Nadu, India. Phytoplasmas were detected in symptomatic leaves of three separate plants using PCR with primer pair fU5/rU3, which amplify an 880-bp fragment of the 16S rRNA region of phytoplasmas. The nucleotide sequence analysis of a 766-bp fragment had 100% identity among the sequence from the three plants (GQ184437) and 96% nucleotide sequence identity with 16S ribosomal RNA gene sequences of eggplant phyllody phytoplasma.

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