A colorimetric method for phosphates is described which employs a molybdatehydrochloric acid solution instead of a molybdate-sulfuric acid solution. This method is not affected by chlorides or by ferric ion up to 15 p. p. m.Fading is less rapid than with most methods. The method is applicable for phosphate determinations in soil fusions, hydrochloric acid extracts of soils, water analyses, oceanographic analyses, plant oxidations in which the sample is taken up in hydrochloric acid, and biological determinations.
SUMMARY
Age‐associated changes in the chemical composition of bovine biceps femoris muscle were studied. Veal muscle had significantly lower Kjeldahl nitrogen and higher moisture contents than muscle from the three older age groups studied. Muscle from veal and from the oldest group (cows, 10 years) possessed less fat than muscle from the two intermediate groups (steers, 1–2 years, and cows, 5 years). A modified procedure for determination of hydroxyproline and its use directly on mean hydrolysates are described. Use of this technique failed to reveal any significant differences in the hydroxyproline content, and presumably the connective‐tissue content, of muscle from the four groups. Warner‐Bratzler shear‐force values of cores from biceps femoris steaks from the three oldest groups indicated that tenderness decreased with age. A method is given for isolation of large quantities of connective tissue from biceps femoris. Chemical analyses of these connective‐tissue residues are presented, and the possibility is discussed that the veal connective tissue contains large amounts of reticulin.
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