The Purpose of this research is: 1) to know and inventories the types of invertebrates which live is in Tikus Island; 2) to develop the biology instructional media of animal course based on the macromedia flash for the 10th grade of high school students, 3) to study the quality of learning based media through the invertebrate’s macromedia, and its assessment on biology teachers. The object of this study utilizes the invertebrate inventory at Tikus island, in which practical survey, interview and explorative descriptive on another types of biota was conducted at Tikus island. The development of product learning was based on macromedia flash research (Research and Development), by implementing the ADDIE Model (Analysis, Design, Development and Production, Implementation, Evaluation). According to the obtained results, there were 18 Species taken from 6 phylum invertebrates. These are Porifera, Coelenterate, Mollusca, Vermes, Echinoderms and Arthropods. The survey showed that based on the validation of linguistic expert (86,4%), validation subject matter experts (83,9 %), validation of media expert (87,8%) the validator eligibility by teachers (84,5%) and the test of the students (86,04%) are all in very good criteria. As seen at the percentage range, it can be inferred that biology media based on macromedia flash of the invertebrate inventory at Tikus island is deemed as very appropriately used by the teachers and students.