Objectives: To evaluating the concentration level of IL-10 in serum of  patients suffering from digestive disorders Blastocystis parasitic infection and compared with control group by using ELISA. Methadology: Case-control study design was conducted on 70 patients (33 males and 37 females) at Al-Sadr medical city and Al-Hakim General Hospital and Al-Sajad Hospital. A control group of 70 participitant (29 males and 41 females) who were without any history of disease were included. Their ages ranged between (6 - ≥ 60) years, during the period from the beginning of November 2021 to  the end of February 2022. Results:  The results showed (48.6%) of culture positive for B.hominis. The results of immunoassay showed mean difference ± standard error difference of IL-10 was (5.878 ± 10.462 Pg/ml) with a 95% confidence interval was (14.809-26.565) in intestinal disorder patients with significant difference (P˂0.001).  Conclusion: Interleukin-10 concentration levels have a significant effect on the results of Blastocystosis patients suffering from digestive disorders.