Background: Human parvovirus B19 (PB19) is a small-sized DNA virus that infects human erythroid progenitor cells causing a sudden cessation of erythropoiesis. PB19 infection constitutes a high risk and may contribute to acute leukemia and worsen the physiological conditions.Objective: Screening acute leukemic patients in Suez Canal University Hospital for anti-human Parvovirus B19 antibodies.Methods: 75 blood samples from acute leukemia patients and 75 blood samples from healthy individuals recruited from Suez Canal University Hospital were subjected to screening for antihuman parvovirus B19 IgM and antihuman parvovirus B19 IgG antibodies by implementing the ELISA technique.Results: The prevalence of antihuman parvovirus B19 IgM and IgG antibodies in patients with acute leukemia was 22.7% and 98.7%, respectively. The prevalence of IgG antibodies against human parvovirus B19 within the control group was 14.7%. Conclusion:This study reported the presence of both types of anti-human parvovirus B19 IgM and IgG antibodies in acute leukemia patients in Suez Canal University hospital, which constitutes a high risk for acute leukemia patients and worsens their clinical blood parameters as well.