Hydro-distilled essential oil (EO) from the leaves of the western Mediterranean and Moroccan endemic plant Tetraclinis articulata was analyzed by GC/MS and examined for its acute toxicity on mice, in order to establish the safe doses. Furthermore, the anti-Inflammatory activity was evaluated based on carrageenan and trauma induced rats paw edema and the antioxidant potential has been investigated using different methods including DPPH radical-scavenging assay, Trolox equivalent antioxidant capacity (TEAC) and Ferric-reducing antioxidant power assay (FRAP). The major identified compounds in GC/MS analysis were bornyl acetate (26.81%), camphor (22.40%) and α-pinene (7.16%), with 25 other minor constituents. No mortalities in acute toxicity were observed, indicating that the LD50 of T. articulata essential oil is highest than 5 g/kg. In the anti-inflammatory test based on chemical and mechanical induced trauma, the EO demonstrated an effective reduce swelling by 64.71 ± 9.38% and 69.09 ± 6.02% respectively obtained 6 h after administration at the dose of 200 mg/kg when compared to the control groups. Moreover in the antioxidant testing battery, T. articulata essential oil showed a promising scavenging effect measured by DPPH, TEAC and ferric-reducing power assays with IC50 values of 12.05 ± 0.24 mg/mL, 8.90 ± 0.17 mg/mL and 0.15 ± 0.01 mg/mL respectively. These results suggest that, the EO from the leaves of T. articulata constitutes a valuable source of anti-inflammatory and antioxidant metabolites. These findings argue for the possible integration of this oil in pharmaceutical, cosmetic and food industries.

Objective: This study was undertaken to justify and validate a very frequent traditional use of a very well-known and widely used plant by a large part of the Moroccan population. It's about Corrigiola telephiifolia, and the activity in question is the diuretic activity. Methods: The aqueous ethanol root extract of corrigiola telephiifolia (200 mg/kg, 400 mg/kg, and 700 mg/kg) was orally administered to rats. The urinary excretion rate and pH, and electrolyte excretion were measured in the urine of saline-loaded rats. Negative control group received only an equivalent volume of distilled water, while the positive control groups received the diuretic drugs hydrochlorothiazide at dose 10 mg/kg. Results: The results showed that hydrochlorothiazide induced significant diuresis and electrolytes excretion at 1, 6 and 24 h after the treatment. Both the higher doses of the extract produced a significant increase in urine volume than the control from the first hour until the end of observation. However, the lowest dose increased significantly only at 24 h after the treatment. With regard to the electrolyte excretion, the tested doses of CTRE which have shown a significant increase in Na + , K + and Clexcretion in comparison to normal control rats, are 400 and 700 mg/kg bw. Conclusion: These findings collectively indicate that the extracts of C. telephiifolia have a potential to induce diuresis markedly, and providing evidence, for its traditional use.

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In this study, the Fourier transform mid-infrared (FT-MIR) spectroscopy technique combined with chemometrics methods was used to monitor adulteration of honey with sugar syrup. Spectral data were recorded from a wavenumber region of 4000–600 cm−1, with a spectral resolution of 4 cm−1. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used for qualitative analysis to discriminate between adulterated and nonadulterated honey. For quantitative analysis, we used partial least-squares regression (PLS-R) and the support vector machine (SVM) to develop optimal calibration models. The use of PCA shows that the first two principal components account for 96% of the total variability. PCA and HCA allow classifying the dataset into two groups: adulterated and unadulterated honey. The use of the PLS-R and SVM-R calibration models for the quantification of adulteration shows high-performance capabilities represented by a high value of correlation coefficients R2 greater than 98% and 95% with lower values of root mean square error (RMSE) less than 1.12 and 1.85 using PLS-R and SVM-R, respectively. Our results indicate that FT-MIR spectroscopy combined with chemometrics techniques can be used successfully as a simple, rapid, and nondestructive method for the quantification and discrimination of adulterated honey.

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