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Objective: During COVID-19, the internet was a prime source for getting relevant updates on guidelines and desirable information. The objective of the present study was to determine the nutritional immunity information seeking behaviour during COVID-19 in India. Design: Google Trends (GTs) data on relevant COVID-19 and nutritional topics were systematically selected and retrieved. Data on newly reported COVID-19 cases were also examined on a daily basis. The cross-correlation method was used to determine the correlation coefficient between the selected terms and daily new COVID-19 cases, and the joint point regression models were utilised to measure monthly percent change in relative search volumes. Setting: Online. Participants: People using google search during period 01-01-2020 to 31-08-2020 in India. Results: The date of peak searches can be attributed to the COVID-19 guidelines announcement dates. All the nutritional terms showed a significant increase in average monthly percentage change. The higher than the average daily rise in COVID-19 cases leads to a higher than average increase in RSVs of nutritional terms with the greatest association after 14 to 27 days. The highest mean relative search volume for nutritional terms was from Southern India (49.34±7.43), and the lowest was from Western India (31.10±6.30). Conclusion: There was a significant rise in the google searches of nutritional immunity topics during COVID-19 in India. The local/regional terms can be considered for better outreach of public health guidelines or recommendations. Further automation of Google Trends using programming languages can help in real-time monitoring and planning various health/nutritional events.

The RP-HPLC method describes developed simple, rapid, specific, selective, accurate and precise method for the simultaneous estimation of Benzthiazide and Triamterene in tablet form. Benzthiazide falls under the category of Thiazide Diuretic and Antihypertensive Agents whereas Triamterene falls under the category of potassium-sparing diuretic and is used in the management of edema associated with congestive heart failure, hepatic cirrhosis with ascites, nephrotic syndrome, and idiopathic edema. RP-HPLC method for Benzthiazide and Triamterene were developed using mobile phase buffer (pH3.5): methanol (70:30v/v), flow rate 1mL/min, injection volume 20μL, temperature 25 ºC detection wavelength 222nm. Linearity range was found to be 5-15μg/ml for Benzthiazide and 10-30μg/ml for Triamterene. LOD and LOQ values were found to be 1.513 and 4.587 μg/ml for Benzthiazide and 0.695 and 2.108 μg/ml for Triamterene respectively. The assay result found to be 99.75 % and 99.97 % for Benzthiazide and triamterene respectively. The developed method was validated according to ICH guidelines.

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