The qualities of seventeen honey samples harvested from the NorthEast areas of Algeria were evaluated by determining the pollen spectrum, pollen number quantity and physicochemical attributes. Pollen analysis can therefore be useful to determine the geographical and botanical origin of honeys. The following determinations were carried out: pH, density, acidity (free, lactone and total), moisture, electrical conductivity, hydroxymethylfurfural, diastase activity, apparent sucrose, and proteins. The results obtained in the present study show the variability of chemical composition of the honey samples. It proved that nine natural honeys are of blossoming origin; suitable for consumption and that one (T5 conferred Bougous) can be used with fine dietetics, it is very rich in pollen which is regarded as protein source. The remainder, eight honeys, were not in conformity with the International Regulatory Standards, their sugar contents (Sucrose) and hydroxymethylfurfural exceeded the International Regulatory Standards Review by the International Honey Commission, this was probably due to use of syrup for the over-feeding the bees during the spring. The sample Bouhachana (G1) had high water content (more than 20%), low density and electrical conductivity higher than 5 µS/cm, which makes it likely to undergo fermentation and degradation. Honeys of Guerguour (T1), Boutheldja (T2) and Bouhadjar (T3) had pH lower than the European standards concerning the quality control of the foodstuffs (exp. honey). These samples are fragile; so we cannot preserve them for a long period. The palynological analysis (qualitative and quantitative) of the harvested samples in the area, showed the absence of a honey obtained from only one melliferous plant. All honeys are polyfloral exits of the several plant species visited by the worker bees during their blossoming periods. Three families are the most represented in the groups of pollen of accompaniment (the secondary pollen ranged between 16-45%) and the pollen considered as rare (minor pollen 3-15%) in the counted total number of the pollen grains in 10 grams of honey. These forager honey families are: Myrtaceae presented by the Eucalyptus, Papilionaceae presented by Hedysarum coronarium which is a forager plant characterized by a very vast surface of development, and Rosaceae represented by orchard and forest species.The pollen grain number counted in honeys is very significant (between 80 000 and 24 832 000pollen grain), what makes it possible to classify the samples analyzed among the categories rich in pollen.
The aim of the present study was to evaluate the quality of 36 samples of different honey type supplied by local producers from Algeria in order to verify its compliance with the standards of Codex Alimentarius and European Union (EU). For that, five physicochemical parameters were analyzed using the HPLC method: hydroxyl-methyl furfural (HMF), sugars, diastase activity and search of antibiotic contamination with streptomycin and tetracycline. The physicochemical analyses of the Algerian honeys show that 56% of samples correspond to Codex standards and 44% not in conformity with the standards required by the Codex Alimentarius and EU, because part of the samples had one or more defects. The percentage not in conformity was due to the high rates of hydroxyl-methyl furfural, sucrose and also to the low enzyme level. Analysis performed by the laboratory to detect residues of tetracycline and streptomycin in honey have revealed insignificant traces of oxytetracycline in two samples of honey (0.03 ppb). From the present study, it is observed that the Algerian honey samples is not completely in agreement with the requirements of international honey standards which could be caused by inappropriate actions during processing and storage steps.
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