Objectives: In this study, we investigated the neuroprotective effect of musk1 after brain ischemia in rats. Methods: The middle cerebral artery occlusion model in rats was established by thread occlusion and reperfusion was performed 90 minutes after ischemia. The reperfusion was performed separately on the first, third, and seventh day of the week after brain ischemia. The ischemia area was detected and the expressions of Arg-1, BCL-2, and lba-1 were detected by immunofluorescence staining. Results: The result of immunofluorescence showed that treatment with musk 50 mg/kg and 100 mg/kg for the first, third, and seventh days significantly improved the neurological function and increased the protein expression of Arg-1 and BCL-2 in the ischemic hemisphere of brains in rats. Also, the ischemic area was reduced. Conclusions: The research results confirms that Mongolian Badanga2 musk in 100 mg/kg doses, has functions of protecting neuro cells, supporting neurogenesis against inflammation, improving neuro tissues regeneration, and reducing ischemic areas through increasing brain tissues Arg-1 and BCL-2 protein's expressions, and reducing lba-1 proteins expressions.

Background Stroke is leading cause of morbidity and mortality in worldwide. Despite valuable progresses in understanding neurological deficits after stroke, its therapeutic options are remaining limited. We aimed to study the neuroprotective effect of musk on cerebral ischemia/reperfusion injury model rats.Methods:In our experiment, we used 180 whore meal breed Wistar rats which weigh 180-220 g and divided these rats into 50 mg/kg of musk, 100 mg/kg of musk, 10 mg/kg of nimodipine, the ischemic-reperfusion groups by filling the midriff of the brain and take the drugs for 7 days in each group. Cerebral ischemia/reperfusion was induced in rats by temporary middle cerebral artery occlusion-reperfusion (MCAO/R) followed by treatment with musk at 50 mg/kg and 100 mg/kg doses. On days 1, 3 and 7 after MCAO/R, TGF-β, BDNF, TrkB and NGF mRNA expressions in the rat brain tissue were quantitatively analyzed using RT-PCR.Results:Musk 50 and 100 mg/kg treated groups brain stroke size were significantly decreased compared with the experimental group at 1, 3 and 7 days. Moreover, brain BDNF, TrkB, NGF and TGF-β mRNA express were not significant difference between experimental and control group. Also 3rd and 7th day, the data indicate that Musk 50 and 100 mg/kg were significantly (p<0,05) effective increasing rats brain BDNF, TrkB, NGF and TGF- β mRNA express in rats with ischemic stroke induced by MCAO/R. Conclusions: The 50 and 100 mg/kg doses of musk lead to increase neuro-protective factors BDNF, TRkB, NGF and TGF- β expression of mRNA in ischemic-reperfusion rat model. It implies that the Mongolian musk supports the neurogenesis of neuronal cell.