Subtle departures from bilateral symmetry (FA) in morphological traits has been used as a measure of developmental stability in populations subjected to genetic and environmental processes as inbreeding, pollution or parasitic infection. Geographic variation of FA was assessed in 19 threespine stickleback (Gasterosteus aculeatus) populations from Min˜o and Limia rivers, from Galicia (NW Spain). This variation was evaluated using four FA indices on five meristic and one morphometric traits. Temporal variation of FA was also assessed using several samples through time in only one population. Significant geographical variation was detected for pectoral fin rays, lateral forks, lateral plates and spine length. Lateral plates, upper gillrakers, pectoral fin rays and spine length showed significant variation through the time. The variation of FA through the time showed a common performance for all characters during the period considered: a slight increase in the intermediate months with a decrease at the end of the period. Symmetric and asymmetric sticklebacks exhibited similar infection, with one exception: symmetric sticklebacks for lower gillrakers of Antela populations exhibited increased parasite infection relative to asymmetric ones. Sticklebacks with extreme phenotypes exhibited much higher levels of asymmetry than modal ones in all samples for all traits.