The acute study revealed no lethal effects at 300mg/kg of HERP, but toxic signs were observed, as HERP had an LD50 of more than 300mg/kg, indicating a warning. The most toxic signals in sub-acute studies were observed in males at a dose of 200mg/kg HERP. These results suggest estrogen-like activity, possibly from the isoflavones in HERP.

Resumo: Nesse trabalho é apresentado a caracterização química do óleo essencial de Siparuna cymosa (Siparunaceae), espécie endêmica da Mata Atlântica, bem como sua avaliação citotóxica frente a linhagem de células tumorais. O óleo foi extraído das folhas secas por hidrodestilação, caracterizado (CG-EM) e submetido a teste de citotóxica através do método colorimétrico usando brometo de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-tetrazólio (MTT) em linhagens de LMA, Leucemia Mielóide Aguda, (THP-1) e LMC, Leucemia Mielóide Crônica (K562). O teor médio de óleo foi de 2,11%, tendo como composto majoritário α-bisabolol (68,9%) e, em quantidade significativas, p-cimen-9-ol (7,9%) e espatulenol (3,7%). Os valores de CI 50 foram de 25,44 ± 1,55 μg mL-1 (LMC) e 30,88 ± 2,45 μg mL-1 (LMA). O óleo essencial apresenta moderada ação citotóxica frente as células leucêmicas, destacando-se a LMA que obteve maior índice de seletividade This work presents the chemical characterization of the essential oil obtained from the Siparuna cymosa (Siparunaceae), an endemic species of the Atlantic Rainforest, as well as its cytotoxicity assessment in the presence of tumor cells. The oil was extracted from dry leaves by hydrodistillation and characterized (GC-FID, GC-MS); and its cytotoxicity was assessed by 3-(4,5-dimethyl-2-thyazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric method in AML, Acute Myeloid Leukemia (THP-1) and CML, Chronic Myeloid Leukemia (K652) cell lines. The average oil content was 2.11% with the major compound being α-bisabolol (68.9%) and, in significant amounts, p-cymen-9-ol (7.9%) and spathulenol (3.7%). The IC 50 obtained were the 25.44 ± 1.55 μg mL-1 (CML), and 30.88 ± 2.45 μg mL-1 (AML). The essential oil exhibits moderate cytotoxic action in the presence of leukemia cells, especially AML, for which the selectivity index was higher.

We present a linear-time algorithm that computes in a given real interval the number of eigenvalues of any symmetric matrix whose underlying graph is unicyclic. The algorithm can be applied to vertex- and/or edge-weighted or unweighted unicyclic graphs. We apply the algorithm to obtain some general results on the spectrum of a generalized sun graph for certain matrix representations which include the Laplacian, normalized Laplacian and signless Laplacian matrices.