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Aims: Weissella cibaria W21, W25 and W42 strains have previously been characterized for their antagonism against a range of foodborne pathogens. However, prior to their use as protective agents, further analyses such as their safety and in situactivity are needed. Methods and Results: The safety of W. cibaria W21, W25 and W42 strains was predicted in silico and confirmed experimentally. Analyses of their genomes using appropriate software did not reveal any acquired antimicrobial resistance genes, nor mobile genetic elements (MGE). The survival of each strain was determined in vitro under conditions mimicking the gastrointestinal tract (GIT). Thus, hemolysis, cytotoxicity and the inflammation capabilities of these strains were respectively determined on blood agar, using a mixture of two cell-lines composed of 50% Caco-2/HT-29 and on the promonocytic human cell line U937. The Weissellastrains were found to be haemolysis-negative, non-cytotoxic and did not induce any inflammation. Furthermore, these strains adhered tightly to intestinal Caco-2 cell-lines and exerted in situ anti-proliferative activity against methicillin resistant Staphylococcus aureus (strain MRSA S1) and Escherichia coli 181, a colistin resistant strain. However, the W. cibariastrains showed low survival rate under simulated GIT conditions in vitro. Conclusion: The unusual LAB-strains Weissella cibaria strains W21, W25 and W42 are safe and endowed with potent antibacterial activities. These strains are therefore good candidates for industrial applications. Significance and Impact of Study: The results of this study allow a better characterization and to gain insights into Weissella strains, which are considered as unusual LAB, but which prompt a growing interest in their bio-functional properties and their potential industrial applications.

The antagonistic Weissella cibaria strains W21, W25 and W42 are characterized in this study for additional beneficial properties. These strains display therefore high scores of adhesion to intestinal cell-lines and capacities to exclude in situ pathogens such as methicillin resistant Staphylococcus aureus (MRSA S1) and Escherichia coli 184, which is resistant to colistin. Further, W. cibaria W21, W25 and W42 were hemolysis-negative, non-cytotoxic towards a mix of eukaryotic Caco2 and HT-29 cell-lines, do not induce inflammation on the promonocytic human cell line U937, but were unable to survive conditions mimicking the human gastrointestinal tract. Their biosafety aspect was addressed in silico and experimentally by studying their blood-hemolysis and susceptibility to antibiotics. To sum up, W. cibaria W21, W25 and W42 have no acquired related antimicrobial resistance genes and mobile genetic elements (MGE) in their genomes. Nevertheless, their intrinsic resistance to vancomycin was established in vitro and confirmed with in silico analyses. Their difficulties to face the gastrointestinal conditions could be overcome by using appropriate delivery systems. Taking all these data together, we claim that W. cibaria W21, W25 and W42 are coming as excellent strains candidates with wide a range of industrial applications and bio-functional properties .

A implantação da Estratégia Saúde da Família (ESF) surgiu com o objetivo de reorganizar o modelo assistencial fundamentado na atenção básica e em conformidade com os princípios do SUS. O presente estudo foi realizado no município de Muritiba/BA, com o objetivo de investigar a percepção da satisfação ao atendimento fisioterapêutico dos usuários cadastrados nas Unidades Básicas da Estratégia Saúde da Família do município. A pesquisa foi realizada por meio da pesquisa de campo, do tipo transversal de caráter analítico, através da aplicação de um questionário direcionado a todos os pacientes cadastrados nas USFs José Maria de Magalhães Neto e Dr. Roberto Penna Fachineppi, submetidos ao tratamento fisioterapêutico oferecido pelos estagiários da FAFIS. A partir da análise quantitativa dos dados, notou-se que os participantes da pesquisa demonstraram-se satisfeitos com o atendimento recebido, considerando-o eficiente. Apesar do curto intervalo de tempo em que esses indivíduos foram submetidos ao atendimento fisioterapêutico, pode-se destacar a suma importância da inserção desse profissional à equipe multiprofissional da ESF, já que este possui habilidades e competências que o permitem intervir nos diversos níveis de assistência à saúde.Palavras-chave: Sistema Único de Saúde, saúde da família, Fisioterapia.

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