Objectives: To assess the diagnostic accuracy of transvaginal ultrasound, aspiration cytology and estradiol content alone and in triad in the discrimination between functional and nonfunctional unilocular ovarian cysts in premenopausal women.Methods: Premenopausal women (n;;; 18) with unilocular ovarian cysts undergoing laparoscopy were recruited.Intracystic fluid was obtained by needle aspiration and subjected to cytology and estradiol content. The cyst wall that was removed during laparoscopy/laparotomy was subjected to histopathology as a gold standard. Cysts were classified into functional or non-functional according to the results of transvaginal ultrasonography, cytology and estradiol content.