This study aimed at in vitro multiplication of Tylophora indica (Burm F.) Merill.via direct shoot regeneration. Initially, screening was done for the analysis of different explants for their competence for direct shoot regeneration. The explants were cultured on media supplemented with eight different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mgl-1) of each cytokinin separately and the concentrations of each cytokinin with highest responses were used in combination with IAA (0.1, 0.25 and 0.50 mgl-1) and IBA (0.1, 0.25 and 0.50 mgl-1). Nodal explants revealed the maximum capacity for direct shoot regeneration among all the explants tested. Following the direct shoot emergence, explants were shifted for subculturing on shoot proliferation media. The medium supplemented with TDZ (1.5 mgl-1) proved effective for achieving optimum number of shoots with appropriate length. The rooting medium supplemented with 0.5 mg/l IBA resulted in maximum number of roots per shoot. Rooted plantlets were successfully hardened in the growth room and later established in the greenhouse. The survival rate of the plants was recorded as 90%. Plants showed no morphological variations.

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