This study aims to determine the motivational orientation and English language attitudes of medical laboratory technology study program students towards learning English. A total of 91 students, in semester 2 of the 2021/2022 academic year, were surveyed using the AMTB (Attitude, Motivation Test Battery), and a questionnaire adapted from Gardner (1985) to determine motivation and language attitudes. Methods: Data were collected by giving a questionnaire measuring motivation and attitudes towards English of students of Medical Laboratory Technology level 1 (one) semester 2, Faculty of Health Sciences, Universitas Perintis Indonesia. Data were analyzed quantitatively and descriptively. Results: Based on research findings, The students studying in the Medical Laboratory Technology Study Program have shown good motivation. From the four aspects in the analysis, it can be seen that students have good motivation. In the integrative aspect, most students already have a good attitude towards learning English. The instrumental aspect thus shows quite positive results. Associated with attitudes towards lecturers who teach is also positive. So it can be concluded that students already have a good attitude and motivation in learning English, but still need development to achieve optimal learning results.